Identification and Quantification of Propolis’s Active Compound in Various Solvents

Abstract

Propolis is a resinous and adhesive substance that collected by honeybees from plant and mixed with various enzymes. The therapeutic effects of propolis such as anticancer, antibacteria, immunomodulator and antiinflammation have already known since several hundred years and had been published in many scientific journals. The therapeutic effects of propolis are sugested due to it’s active compound such as CAPE, artepillin C, quercetin and hesperidine. Different extraction methods especially realted with the using of solvent influence the active component extracted from raw propolis. The aim of this study was to identify and quantify the CAPE, artepillin C, quercetin and hesperidine compound in propolis sample collected from beeskeeping in Kediri, East Java. The propolis extracted by maseration technique using different solvent such as methanol, ethyl acetate and N-heksan. Identification and quantification were performed using LC-MS/MS and LC-Quan software respectively. Various results were shown, in which the highest concentration for CAPE and quercetin were obtained from methanol. Furthermore, hesperidine and artepillin-C were reach the highest concentration in ethyl acetate and n-hexane respectively. In conclusion, methanol was the better solvent than ethyl acetat and Nheksan.